| MGQuest |
Description
GemTaq™ Hot Start 5xMaster Mix was developed for ultimate convenience, time conservation and greater productivity. It is a ready-to-
use 5x PCR reaction mix containing GemTaq™ Hot Start DNA Polymerase, dNTPs, MgCl2 and reaction buffer at optimal concentrations
for efficient amplification of DNA templates. Users of the GemTaq™ Hot Start 5xMaster Mix only need to add water, primers and
template to perform PCR assays. Use of the GemTaq™ Hot Start 5xMaster Mix provides robust yields, high specificity and sensitivity
of PCR amplifications within a short reaction time. The superior performance of this mix is due to properties of GemTaq™ Hot Start
DNA Polymerase that is a heat-activated high-performance proprietary formulation of GemTaq™ DNA Polymerase.
The modified polymerase activity is restored during the initial denaturation step (94-95 °C for 3 min.) allowing for a hot-start PCR.
Therefore GemTaq™ Hot Start DNA Polymerase provides improved specificity and very high PCR sensitivity by eliminating the
presence of non-specifics products.
Advantages
• Ultimate convenience, time conservation and higher productivity.
• Ultra-high specificity.
• Fast activation.
• Greater efficiency and sensitivity in a wide range of PCR assays within a short reaction time.
• Recommended for low copy assays.
• Amplifies fragments up to 5 kb.
• Unbeatable value in terms of cost per unit and performance.
Applications
• Hot start PCR.
• Low-copy target.
• High throughput PCR applications
• Complex genomic templates.
• TA cloning.
FOR LABORATORY USE ONLY
GemTaq™ Hot Start 5xMaster Mix was developed for ultimate convenience, time conservation and greater productivity. It is a ready-to-
use 5x PCR reaction mix containing GemTaq™ Hot Start DNA Polymerase, dNTPs, MgCl2 and reaction buffer at optimal concentrations
for efficient amplification of DNA templates. Users of the GemTaq™ Hot Start 5xMaster Mix only need to add water, primers and
template to perform PCR assays. Use of the GemTaq™ Hot Start 5xMaster Mix provides robust yields, high specificity and sensitivity
of PCR amplifications within a short reaction time. The superior performance of this mix is due to properties of GemTaq™ Hot Start
DNA Polymerase that is a heat-activated high-performance proprietary formulation of GemTaq™ DNA Polymerase.
The modified polymerase activity is restored during the initial denaturation step (94-95 °C for 3 min.) allowing for a hot-start PCR.
Therefore GemTaq™ Hot Start DNA Polymerase provides improved specificity and very high PCR sensitivity by eliminating the
presence of non-specifics products.
Advantages
• Ultimate convenience, time conservation and higher productivity.
• Ultra-high specificity.
• Fast activation.
• Greater efficiency and sensitivity in a wide range of PCR assays within a short reaction time.
• Recommended for low copy assays.
• Amplifies fragments up to 5 kb.
• Unbeatable value in terms of cost per unit and performance.
Applications
• Hot start PCR.
• Low-copy target.
• High throughput PCR applications
• Complex genomic templates.
• TA cloning.
FOR LABORATORY USE ONLY
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Prices valid for US customers only
| 2007-2012 MGQuest. All rights reserved. |
GemTaq™ Hot Start Amplification Family
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