| MGQuest |
Description
GemTaq™ Hot Start 5xMaster Mix was developed for ultimate convenience, time conservation and greater productivity. It is a ready-to-
use 5x PCR reaction mix containing GemTaq™ Hot Start DNA Polymerase, dNTPs, MgCl2 and reaction buffer at optimal concentrations
for efficient amplification of DNA templates. Users of the GemTaq™ Hot Start 5xMaster Mix only need to add water, primers and
template to perform PCR assays. This mix also provides a direct loading of the PCR samples onto gels without the need to add a loading
sample buffer. It contains a compound that increases sample density for electrophoresis and two dyes (Red and Yellow) that separate
during electrophoresis and allow easy monitoring. The Red and Yellow dyes migrate at the same rate as 800-1000bp DNA fragments and
20-30bp DNA fragments in a 1% agarose gel, respectively.
Use of the GemTaq™ Hot Start 5xMaster Mix provides robust yields, high specificity and sensitivity of PCR amplifications within a
short reaction time. The superior performance of this mix is due to properties of GemTaq™ Hot Start DNA Polymerase that is a heat-
activated high-performance proprietary formulation of GemTaq™ DNA Polymerase. The modified polymerase activity is restored during
the initial denaturation step (94-95 °C for 3 min.) allowing for a hot-start PCR. Therefore GemTaq™ Hot Start DNA Polymerase
provides improved specificity and very high PCR sensitivity by eliminating the presence of non-specifics products.
Advantages
• Ultimate convenience, time conservation and higher productivity.
• Ultra-high specificity.
• Fast activation.
• Greater efficiency and sensitivity in a wide range of PCR assays within a short reaction time.
• Recommended for low copy assays.
• Amplifies fragments up to 5 kb.
• Unbeatable value in terms of cost per unit and performance.
Applications
• Hot start PCR.
• Low-copy target.
• High throughput PCR applications
• Complex genomic templates.
• TA cloning.
FOR LABORATORY USE ONLY
GemTaq™ Hot Start 5xMaster Mix was developed for ultimate convenience, time conservation and greater productivity. It is a ready-to-
use 5x PCR reaction mix containing GemTaq™ Hot Start DNA Polymerase, dNTPs, MgCl2 and reaction buffer at optimal concentrations
for efficient amplification of DNA templates. Users of the GemTaq™ Hot Start 5xMaster Mix only need to add water, primers and
template to perform PCR assays. This mix also provides a direct loading of the PCR samples onto gels without the need to add a loading
sample buffer. It contains a compound that increases sample density for electrophoresis and two dyes (Red and Yellow) that separate
during electrophoresis and allow easy monitoring. The Red and Yellow dyes migrate at the same rate as 800-1000bp DNA fragments and
20-30bp DNA fragments in a 1% agarose gel, respectively.
Use of the GemTaq™ Hot Start 5xMaster Mix provides robust yields, high specificity and sensitivity of PCR amplifications within a
short reaction time. The superior performance of this mix is due to properties of GemTaq™ Hot Start DNA Polymerase that is a heat-
activated high-performance proprietary formulation of GemTaq™ DNA Polymerase. The modified polymerase activity is restored during
the initial denaturation step (94-95 °C for 3 min.) allowing for a hot-start PCR. Therefore GemTaq™ Hot Start DNA Polymerase
provides improved specificity and very high PCR sensitivity by eliminating the presence of non-specifics products.
Advantages
• Ultimate convenience, time conservation and higher productivity.
• Ultra-high specificity.
• Fast activation.
• Greater efficiency and sensitivity in a wide range of PCR assays within a short reaction time.
• Recommended for low copy assays.
• Amplifies fragments up to 5 kb.
• Unbeatable value in terms of cost per unit and performance.
Applications
• Hot start PCR.
• Low-copy target.
• High throughput PCR applications
• Complex genomic templates.
• TA cloning.
FOR LABORATORY USE ONLY
|
Prices valid for US customers only
| 2007-2012 MGQuest. All rights reserved. |
GemTaq™ Hot Start Amplification Family
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