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Description
GemTaq™ Hot Start DNA Polymerase is a heat-activated high-performance proprietary formulation of GemTaq™ DNA Polymerase.
The modified polymerase activity is restored during the initial denaturation step (94-95 °C for 3 min.) allowing for a hot-start PCR.
Therefore GemTaq™ Hot Start DNA Polymerase provides improved specificity and very high PCR sensitivity by eliminating the
presence of non-specifics products. It has an elongation rate higher than regular Taq DNA Polymerase, resulting in robust yields and
greater sensitivity of PCR amplifications within a short reaction time. GemTaq™ Hot Start DNA Polymerase has 5’→3’ DNA
polymerase activity and 5’→3’ exonuclease activity. The enzyme can amplify fragments of up to 5 kb in length from genomic DNA and
longer fragments from Lambda DNA. GemTaq™ Hot Start DNA Polymerase is supplied at 5u/µl and comes with an optimized
5xReaction buffer. The purified enzyme has no detectable endonuclease or exonuclease activity.
Advantages
• Ultra-high specificity.
• Fast activation.
• Greater efficiency and sensitivity in a wide range of PCR assays within a short reaction time.
• Recommended for low copy assays.
• Amplifies fragments up to 5 kb.
• Unbeatable value in terms of cost per unit and performance.
Applications
• Hot start PCR.
• Low-copy target.
• High throughput PCR applications
• Complex genomic templates.
• TA cloning.
FOR LABORATORY USE ONLY
GemTaq™ Hot Start DNA Polymerase is a heat-activated high-performance proprietary formulation of GemTaq™ DNA Polymerase.
The modified polymerase activity is restored during the initial denaturation step (94-95 °C for 3 min.) allowing for a hot-start PCR.
Therefore GemTaq™ Hot Start DNA Polymerase provides improved specificity and very high PCR sensitivity by eliminating the
presence of non-specifics products. It has an elongation rate higher than regular Taq DNA Polymerase, resulting in robust yields and
greater sensitivity of PCR amplifications within a short reaction time. GemTaq™ Hot Start DNA Polymerase has 5’→3’ DNA
polymerase activity and 5’→3’ exonuclease activity. The enzyme can amplify fragments of up to 5 kb in length from genomic DNA and
longer fragments from Lambda DNA. GemTaq™ Hot Start DNA Polymerase is supplied at 5u/µl and comes with an optimized
5xReaction buffer. The purified enzyme has no detectable endonuclease or exonuclease activity.
Advantages
• Ultra-high specificity.
• Fast activation.
• Greater efficiency and sensitivity in a wide range of PCR assays within a short reaction time.
• Recommended for low copy assays.
• Amplifies fragments up to 5 kb.
• Unbeatable value in terms of cost per unit and performance.
Applications
• Hot start PCR.
• Low-copy target.
• High throughput PCR applications
• Complex genomic templates.
• TA cloning.
FOR LABORATORY USE ONLY
GemTaq™ Hot Start Amplification Family
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