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SNPase Hot Start DNA Polymerase
Description
SNPase Hot Start is a thermostable DNA polymerase designed for a single-nucleotide polymorphism (SNP) detection. This enzyme has
only 5'-3' polymerase activity and lacks exonuclease activity. SNPase Hot Start DNA Polymerase provides very high enzymatic
specificity and high fidelity incorporation of dNTPs and ddNTPs that is 10-15 times higher than a regular Taq DNA Polymerase. The
enzyme has a modified polymerase activity that is restored during the initial denaturation step allowing for a hot-start PCR and eliminates
the presence of non-specifics products. SNPase Hot Start DNA polymerase is recommended for SNP genotyping by allele-specific PCR
(AS-PCR), allele-specific primer extension (AS-PEX) and mini sequencing procedures. The enzyme also can be used for high fidelity
amplification of short DNA fragments up to 500 bp from Human genomic DNA and up to 1000 bp from plasmid DNA. SNPase Hot
Start DNA Polymerase is supplied at 12 u/µl and comes with an optimized 5xReaction buffer (with 12.5 mM Mg2+) and 100 mM
MgCl2. The purified enzyme has no detectable endonuclease or exonuclease activity.
Features
• High sensitivity to mismatches at 3’-end of primers.
• High fidelity of dNTPs and ddNTPs incorporation.
• Lack of 5’-3’ and 3’-5’exonuclease activity.
• High specificity, low level of background PCR.
• Fast activation (5-10 sec, 95°C).
• TA cloning.
Applications
• SNP- genotyping, allele specific PCR, allele specific primer extension, mini-sequencing.
• High fidelity PCR (up to 1000 bp).
• Multiplex PCR.
• Real Time PCR with intercalating dyes (SYBR Green, Eva Green).
Limitations
• SNPase can’t be used in TaqMan assay due to the lack of 5’- exonuclease activity.
• SNPase can’t be used for effective amplification of templates longer than 1000 bp.
FOR LABORATORY USE ONLY
SNPase Hot Start is a thermostable DNA polymerase designed for a single-nucleotide polymorphism (SNP) detection. This enzyme has
only 5'-3' polymerase activity and lacks exonuclease activity. SNPase Hot Start DNA Polymerase provides very high enzymatic
specificity and high fidelity incorporation of dNTPs and ddNTPs that is 10-15 times higher than a regular Taq DNA Polymerase. The
enzyme has a modified polymerase activity that is restored during the initial denaturation step allowing for a hot-start PCR and eliminates
the presence of non-specifics products. SNPase Hot Start DNA polymerase is recommended for SNP genotyping by allele-specific PCR
(AS-PCR), allele-specific primer extension (AS-PEX) and mini sequencing procedures. The enzyme also can be used for high fidelity
amplification of short DNA fragments up to 500 bp from Human genomic DNA and up to 1000 bp from plasmid DNA. SNPase Hot
Start DNA Polymerase is supplied at 12 u/µl and comes with an optimized 5xReaction buffer (with 12.5 mM Mg2+) and 100 mM
MgCl2. The purified enzyme has no detectable endonuclease or exonuclease activity.
Features
• High sensitivity to mismatches at 3’-end of primers.
• High fidelity of dNTPs and ddNTPs incorporation.
• Lack of 5’-3’ and 3’-5’exonuclease activity.
• High specificity, low level of background PCR.
• Fast activation (5-10 sec, 95°C).
• TA cloning.
Applications
• SNP- genotyping, allele specific PCR, allele specific primer extension, mini-sequencing.
• High fidelity PCR (up to 1000 bp).
• Multiplex PCR.
• Real Time PCR with intercalating dyes (SYBR Green, Eva Green).
Limitations
• SNPase can’t be used in TaqMan assay due to the lack of 5’- exonuclease activity.
• SNPase can’t be used for effective amplification of templates longer than 1000 bp.
FOR LABORATORY USE ONLY
Prices valid for US customers only
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